Plasmid
leghemoglo

Part:BBa_K4232009

Designed by: Songyuan Kong   Group: iGEM22_BIT-China   (2022-10-03)


This is a plasmid used to transport oxygen within a cell

This is a plasmid consisting of legumoglobin and p5T7,it is used to transport oxygen within cells.The specific working principle is show in the BBa_K4232001.The specific construction of the legumoglobin is shown in Figure 1.

Figure 1.legumoglobin plasmid diagram










Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 5135
    Illegal suffix found in sequence at 443
    Illegal PstI site found at 116
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5135
    Illegal NheI site found at 2867
    Illegal SpeI site found at 444
    Illegal PstI site found at 116
    Illegal PstI site found at 458
    Illegal NotI site found at 451
    Illegal NotI site found at 5141
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5135
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 5135
    Illegal suffix found in sequence at 444
    Illegal PstI site found at 116
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 5135
    Illegal XbaI site found at 5150
    Illegal SpeI site found at 444
    Illegal PstI site found at 116
    Illegal PstI site found at 458
    Illegal NgoMIV site found at 2761
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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